Study of the influence of gluten on behavioral and biochemical parameters in animals submitted in models of neuropsychiatric diseases

Gluten and behavioral and biochemical parameters in animals submitted in models of neuropsychiatric diseases

Abstract

Objectives: The presence of gluten in the diet can trigger several pathophysiological processes that culminate in neuropsychiatric diseases in addition to gastrointestinal problems. In the so-called “non-celiac gluten-sensitive”, the literature data are divergent. Therefore, the objective of the present work was to evaluate the effect of treatments of different diets related to gluten content, on behavioral and biochemical parameters. Methods: C56bl/6j mice (female, N:7-10) were subjected to behavioral tests for neuropsychiatric disorders. The animals were divided into groups: G1/food enriched with 75% gluten (DEG), G2/normal diet (Nuvilab®-DNN) and G3/gluten-free diet (DIG). For the depression and anxiety tests, two additional groups were introduced (controls - positive) that received respectively Fluoxetine (20mg/kg mg), Diazepam (0.75mg/kg and galanthamine (4mg kg/kg). The animals were submitted to behavioral tests: Open field, Suspension by the tail (TSC), Elevated plus maze (LCE) and Inhibitory Avoidance (TEI). ALT, AST, fasting glucose, total cholesterol, HDL, TG) Results: The results showed that DEG triggered anxiety and depression behaviors in animals, in addition to memory deficits, it also promoted hyperglycemia,   increase in body weight,  decreased HDL and increased LDL. may lead to a malfunction of liver functions (AST and ALT) Conclusions: The results together confirm the deleterious effects caused by gluten.